Migration Inhibitory Factor, Recombinant, Human (Macrophage MIF, GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor)
Product Sizes
2ug
£504.00
M3895-07-2UG
About this Product
- SKU:
- M3895-07
- Extra Details:
- Macrophage migration inhibitory activity originally identified 30 years ago based on the ability to prevent the migration of guinea pig macrophages out of capillary tubes in vitro. A number of cytokines, including interferon-g and IL-4, exhibit macrophage migration inhibitory activity. A cDNA clone was isolated from a lectin-stimulated T-cell hybridoma cDNA library and reported to encode a protein with macrophage migration inhibitory activity. This protein was referred to as human migration inhibitory factor (MIF). MIF cDNA encodes a 115 amino acid residue polypeptide that lacks conventional hydrophobic leader sequence. MIF is expressed in many tissues and in all cell lines examined to date. MIF was also discovered as a growth factor-induced delayed early response gene DER6. Recently, MIF was reported to be a secreted cytokine that exerts a central and wide ranging role in host inflammatory responses. Evidence for a receptor of this has not been reported. E. coli-expressed MIF lacks macrophage migration inhibitory activity and is recommended for use as a calibrator for MIF immunoassays. E. coli-derived recombinant protein corresponding to Pro2-Ala115 from human MIF, with an N-terminal Met Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS, > 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 12.5
- Physical State:
- Supplied as a lyophilized powder from 100mM Tris, pH 8.0 and 50ug BSA/ug protein. Reconstitute with sterile 200ul PBS, > 0.1% BSA or HSA.
- Purity:
- ≥97% as determined by N-terminus analysis and SDS-PAGE (silver stain). Endotoxin: <0.10EU/ug (LAL)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
