Microphthalmia (Transcription Factor, MITF)
Product Sizes
100ug
£953.00
M3891-04A-100UG
About this Product
- SKU:
- M3891-04A
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although is has a predicted MW of 58kD, it runs anomalously in SDS-PAGE at 57-66kD. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526aa in length. It contains a bHLH DNA binding region (aa309-369) and a Leu-zipper domain (aa374-395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa294-299. One variant has a deletion of aa139-194, a second has an 11aa substitution for aa1-118, and three others show variable substitutions over aa1-35. Over aa119-289, human MITF-A1 shares 96% aa identity with mouse MITF-A1. Applications: Suitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- E. coli-derived, recombinant protein corresponding to Val119-Lys289 from human MITF. .Species sequence homology: mouse (96%).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human MITF.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
