b2-Microglobulin (b2M)
Product Sizes
100ul
£927.00
M3890-55-100UL
About this Product
- SKU:
- M3890-55
- translate.label.attr.clone:
- 3H36
- Clonality:
- Monoclonal
- Extra Details:
- Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer’s disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600D (human 119aa, chromosome 15). B2M associates with the heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. B2M is ~12kD. Western Blot positive control (M3890-55B) should be run to identify the appropriate B2M band and find optimum antibody dilutions. ELISA: 1:100,000 using 50-100ng control antigen/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Purified human B2M
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Ascites
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human b2-microglobulin. Species Crossreactivity: Crossreactivity is expected with mouse and rat B2M.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
