MHC Class 2, I-A, Rt1.Bu (PE)
Product Sizes
100Tests
£723.00
M3887-49L-100TESTS
About this Product
- SKU:
- M3887-49L
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- OX-3
- Clonality:
- Monoclonal
- Extra Details:
- The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Rat thymocyte membrane glycoproteins.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Purity:
- Purified by Ion Exchange chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats, but not RN, DA or PVG/c strains. The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes Ia antigens on B cell, dendritic cells and certain epithelial cells. It is par
- Source:
- rat
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
