Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6) (PE/CY5)
Product Sizes
100ug
£466.00
L7715-05E-100UG
About this Product
- SKU:
- L7715-05E
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- D7
- Clonality:
- Monoclonal
- Extra Details:
- Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes.1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.1, 8, 9 Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses,9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.3, 13 Applications • Flow cytometry 1, 4-7 • Immunoprecipitation 1 • Western blotting 1, 2 • Immunohistochemistry (frozen sections) • In vitro lymphocyte activation 9-12 Recommended Dilution: Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
- Host:
- Rat
- Immunogen:
- IL-2-dependent mouse T-cell line CTL-L
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18kD.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
