LIR-8 (Leukocyte Immunoglobulin-like Receptor 8, CD85c)
Product Sizes
100ug
£889.00
L2637-45-100UG
About this Product
- SKU:
- L2637-45
- Application:
- Flow Cytometry, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Flow Cytometry: 2.5ug/10e5 cells. Validated using human peripheral blood monocytes and granulocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Arg18-His456 from human LIR-8, expressed in NS0 cells.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human LIR-8, Shows <20% crossreactivity with rhLIR-4, ~10% with rhILT5, rhLIR-5, rhLIR-6, and rhLILRA5 and <5% with rhILT2, rhILT4, and rhLIR-7.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
