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Antibodies

Keratin, pan (AlexaFluor®647)

Product Sizes
100ul
£735.00
K0199-92A-100UL
About this Product
SKU:
K0199-92A
Application:
Flow Cytometry, Immunohistochemistry, Immunofluorescence
translate.label.attr.clone:
7H35(C11)
Clonality:
Monoclonal
Extra Details:
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:50-1:200 Immunofluorescence (IF-IC): 1:800-1:3200 Immunofluorescence (Frozen): 1:100-1:400 Flow Cytometry (Fixed/Permeabilized): 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light.
Host:
Mouse
Immunogen:
Cytoskeleton preparation from A431 cells.
Isotype:
IgG1
Physical State:
Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 647.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes endogenous levels of total human keratins 4, 5, 6, 8, 10, 13 and 18. Does not crossreact with other keratins. Species Crossreactivity: Rat, mouse and monkey.
Source:
human
Storage Conditions:
4[o]C Do not freeze
Type:
Antibodies:Monoclonal Antibody