Kallikrein B1 (KLKB1, Plasma Kallikrein)
Product Sizes
100ug
£832.00
K0005-28B-100UG
About this Product
- SKU:
- K0005-28B
- Application:
- Western Blot
- translate.label.attr.clone:
- 7H97
- Clonality:
- Monoclonal
- Extra Details:
- Kallikrein B1 (KLKB1) is a serine protease synthesized in the liver and circulated in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. It plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation. Mouse KLKB1 precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638). Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain. The amino acid sequence of mouse KLKB1 is 90%, 77% and 76% identical to that of rat, canine and human. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under non-reducing conditions only Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gly20-Ala638 from mouse Kallikrein B1, expressed in NS0 cells
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma tissue supernatant
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Kallikrein B1. Does not recognize rhKLKB1, rhKallikrein 1,3, 4, 5, 7, 8, 10, 11, 12, 13, 14, 15, rmHGFA, rhFactor VI, rh Factor X, rhFactor XI, rhThrombin or rhuPA.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
