JAK1 (Janus Kinase 1)
Product Sizes
100ug
£869.00
J0900-06A-100UG
About this Product
- SKU:
- J0900-06A
- Application:
- Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 7H242
- Clonality:
- Monoclonal
- Extra Details:
- Janus Kinase 1 (JAK1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of JAK1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. JAKs activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. JAK1 is required for the activation of STAT1 and STAT2 in response to interferon alpha. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Flow Cytometry: 25ug/ml; 10ul labels 1-5 x 10e5 Jurkat cells in a total reaction volume ≤ 200ul. For intracellular staining, cells must first be fixed and permeabilized using 2% paraformaldehyde and ice-cold methanol. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunocytochemistry: 10ug/ml detects JAK1 in HeLa cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted J0900-06A followed by Rhodamine Red-coupled anti-rat IgG in the dark. Between each step, cells were washed with PBS, BSA, Triton X-100. Immunohistochemistry: 25ug/ml detects JAK1 in paraffin-embedded normal human skin tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- E. coli-derived recombinant human JAK1 (aa20-274, Accession #AAA36527)
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human JAK1 at ~130kD. Species Crossreactivity: rat and mouse
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
