Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8) (APC)
Product Sizes
100Tests
£806.00
I8439-08G-100TESTS
About this Product
- SKU:
- I8439-08G
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 7H34
- Clonality:
- Monoclonal
- Extra Details:
- Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, hIL-17 shows 72% and 63% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kB and co-stimulation of T cell proliferation. The non-glycosylated, E. coli-expressed rhIL-17 has been shown to be active on human as well as mouse cell lines. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. 10ul labels up to1x10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ile20-Ala155 of human IL-17 expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human IL-17. Species Crossreactivity: recombinant human IL-17A/IL-17F ~40%. Does not crossreact with recombinant human IL17B, -C, -D -E, -F, recombinant mouse IL17, or recombinant canine IL-17.
- Source:
- human
- Storage Conditions:
- 4[o]C Do not freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
