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Antibodies

Interleukin 7 Receptor alpha (IL7Ra, CD127, CDw127, L7RA, IL-7R-alpha, ILRA)

Product Sizes
100ug
£876.00
I8429-27J-100UG
About this Product
SKU:
I8429-27J
Application:
Western Blot
Clonality:
Polyclonal
Extra Details:
IL-7 R-alpha (IL-7 receptor alpha; also CD127) is a 60-70kD member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R-alpha heterodimerizes with both the gc chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R-alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R-alpha is a 437aa type I transmembrane glycoprotein. It contains a 219aa extracellular region (aa21-239) that possesses one type III fibronectin domain (aa129-222), and a 193aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Sheep
Immunogen:
Purified, NS0-derived, recombinant rat IL-7 R alpha, extracellular domain.
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes rat IL-7 R-alpha.
Source:
rat
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody