Interleukin 6 Receptor (CD126, IL-6R) (Carboxyfluorescein)
Product Sizes
100Tests
£665.00
I8428-39L2-100TESTS
About this Product
- SKU:
- I8428-39L2
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 8H112
- Clonality:
- Monoclonal
- Extra Details:
- The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80kD component receptor that binds IL-6 with low affinity (IL-6 R) and a signal-transducing component of 130kD (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R and gp130 have been cloned, sequenced, and expressed . A soluble form of the IL-6 R has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R. No naturally-occurring mRNA encoding a truncated form of the IL-6 R has been reported. Soluble forms of human and murine IL-6 Rs have been constructed, however, by insertion of termination codons into the regions of the IL-6 R cDNAs encoding the external portions of the receptors prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R complex to membrane-bound gp130. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: Carboxyfluorescein conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha, Leu20-Asp339.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Carboxyfluorescein.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human IL-6R. Does not react with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
