Interleukin 6 (IL-6)
Product Sizes
100ug
£607.00
I8428-25A-100UG
About this Product
- SKU:
- I8428-25A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Neutralization: 0.2-0.4ug/ml yields one-half maximal inhibition [ND50] of the biological activity (1ng/ml). Direct ELISA: 0.5ug/ml (100ul/well). using I8428-03B1 as a detection antibody detects 0.2-0.4ng/ml of recombinant human IL-6. Western Blot: 0.1-0.2ug/ml. The detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Interleukin 6 (IL-6), expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Do not vortex.
- Purity:
- Purified by immunoaffinity chromatography. Endotoxin: ≤1EU/ug.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Interleukin-6 (IL-6).
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
