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Antibodies

Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Product Sizes
500ug
£908.00
I8427-15C-500UG
About this Product
SKU:
I8427-15C
Application:
Flow Cytometry, Western Blot
translate.label.attr.clone:
6A422 (TRFK5)
Clonality:
Monoclonal
Extra Details:
IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation. This clone may be used to neutralise IL-5 bioactivity in a functional assay (1,2). Removal of sodium azide is recommended prior to use in functional assays. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: Membrane permeabilization is required for this application. Optimal dilution determined by the researcher. Flow Cytometry: Recommended Secondary Reagents: F(ab')2 Goat anti rat IgG:FITC (mouse adsorbed) F(ab')2 Goat anti rat IgG:RPE (mouse adsorbed) Western Blot: Recommended Secondary Reagents: Goat anti rat IgG:HRP (mouse adsorbed) Recommended Negative Controls: Rat IgG1 Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Rat
Immunogen:
Purified natural mouse IL-5.
Isotype:
IgG1
Physical State:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity:
Purified by Protein A affinity chromatography from ascites.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse Interleukin 5 (IL-5). Species Crossreactivity: human, porcine and guinea pig.
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody