Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
Product Sizes
2ug
£364.00
I7663-2UG
About this Product
- SKU:
- I7663
- Extra Details:
- IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Human IL-1alpha is a 18.0kD protein containing 159 amino acid residues. Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is ≤ 0.001ng/ml, corresponding to a specific activity of ≥ 1x10e9 units/mg. Amino Acid Sequence: SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 18
- Physical State:
- Supplied as a lyophilized powder from 10mM Tris, pH 8.0, 75mM sodium chloride. Reconstitute in sterile ddH2O to a concentration of 0.1-1mg/ml.
- Purity:
- ≥98% by SDS-PAGE and HPLC. Endotoxin: ≤ 0.1ng/ug (1EU/ug)
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
