Interleukin 33 (IL-33)
Product Sizes
100ug
£978.00
I7663-97A-100UG
About this Product
- SKU:
- I7663-97A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Applications: Suitable for use in ELISA, Immunocytochemistry, Neutralization and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 0.2-0.8ug/ml as capture antibody Immunocytochemistry: 5-15ug/ml using immersion fixed human PBMCs treated with PMA and calcium ionomycin Neutralization: Measured by its ability to neutralize IL33induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.753.0ug/ml in the presence of 1 ng/mL recombinant human IL33 and suboptimal amounts of mouse CD3-epsilon monoclonal antibody. Western Blot: 0.1ug/ml using rhIL-33 as sample Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant human IL-33 (aa112-270)
- Isotype:
- IgG
- Physical State:
- Supplied as a powder lyophilized from a from a 0.2um filtered solution in PBS containing trehalose. Reconstitute to 0.2mg/ml with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human IL-33. Less than 0.05% cross-reactivity with recombinant mouse IL-33 is observed in ELISA.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
