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Antibodies

Interleukin 2 (IL-2) (Biotin)

Product Sizes
500ug
£607.00
I7663-27K2-500UG
About this Product
SKU:
I7663-27K2
Application:
Immunoprecipitation, Western Blot
translate.label.attr.clone:
JES6-1A12
Clonality:
Monoclonal
Extra Details:
Applications: ELISA: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-2 protein levels.1-3 Purified antibody may be paired with biotinylated antibody (Cat. No. I7663-27M5) as the detection antibody, with purified recombinant mouse IL-2 as the standard. Or, conversely, biotinylated antibody may be paired with purified JES-5H4 antibody (Cat. No. I7663-27M4) as the capture antibody. Western Blot: Useful for immunoprecipitation and Western blot applications.1 For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100ng/lane of recombinant mouse IL-2 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Rat
Immunogen:
E. coli-expressed recombinant mouse IL-2
Isotype:
IgG2a,k
Physical State:
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Purity:
Purified
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse Interleukin-2 (IL-2).
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody