Insulin-Like Growth Factor Binding Protein 2 (IGFBP2, BP2, IBP2) (Not for Export EU)
Product Sizes
100ul
£704.00
I7661-16G6-100UL
About this Product
- SKU:
- I7661-16G6
- Application:
- Immunoprecipitation, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- The insulin like growth factors (IGFs) are the major growth-promoting factors in the plasma. IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation. The A and B domains of IGFs are identical to insulin. IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity. The IGF-IIR lacks the kinase activity and is actually identical to the mannose-6-phophate receptor. Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs). At least 6 related IGFBPs (IGFBP1-6) have been well characterized. Recently, IGFBP-7/Mac25/prostacyclin-stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges. These proteins are present in plasma in high concentration as compared to the membrane IGFRs. Therefore, IGFBPs have the potential to modulate the IGF action. Applications: Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 (ECL). IGFBP-2 is ~34kD. Concentrated T98 cell serum free culture medium or other cells can be used as positive control. ELISA: 1:10,000 using 50-100ng control peptide/well. Immunoprecipitation: 5-10ul per 500ul of concentrated serum free medium. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Purified bovine IGFBP2 (36kD).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes bovine IGFBP2. Species Crossreactivity: human, mouse, rat and primate. Minimal crossreactivity <0.1% with IGFBP-1, -4, -5, and IGFBP-3, <0.5%.
- Source:
- bovine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
