Immunoglobulin-Like Transcript 2 (ILT2, CD85j) (APC)
Product Sizes
100Tests
£806.00
I7502-70-100TESTS
About this Product
- SKU:
- I7502-70
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 7H15
- Clonality:
- Monoclonal
- Extra Details:
- The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1-3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex, which also include the genes encoding KIRs (4). With the exception of ILT6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3). One subset of the ILT receptors (referred to as subfamily B of the LIRs) has long cytoplasmic tails containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events by recruiting SH2-containing protein tyrosine phosphatase-1. Another subset of the ILT receptors (referred to as subfamily A of the LIRs) contains activating receptors with short cytoplasmic regions that lack signal transduction motifs. These receptors contain a basic arginine residue within their transmembrane domains that allows association with Fcγ R, an immunoreceptor tyrosine- based activation motif (ITAM)-bearing signal adapter protein (1-3). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly24-His458 from human ILT2/CD85j, expressed in NSO cells.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in saline, <0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human ILT2/CD85j. In Western Blot, shows no crossreactivity with recombinant human ILT4, ILT5, LIR6 or LIR8.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
