ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein Gl50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7 H2 ICOS Ligand)
Product Sizes
500ug
£1004.00
I0650-01H-500UG
About this Product
- SKU:
- I0650-01H
- Application:
- Western Blot
- translate.label.attr.clone:
- 8H103
- Clonality:
- Monoclonal
- Extra Details:
- ICOS Ligand, also known as B7-related protein 1 (B7RP-1), is a costimulatory molecule that serves as a ligand for ICOS (Inducible Co-Stimulator). Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Neutralization: 1-4ug/ml yields one-half maximal inhibition (ND50) of the cell surface ICOS Ligand in the presence of 20ng/ml of anti-human CD3 and 3ug/ml of recombinant human B7-H2/Fc, using B7-H2-dependent proliferation of PHA activated human T cells as an assay. Western Blot: 1ug/ml with recombinant human B7-H2 Fc Chimera Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp19-Ser258 from human ICOS Ligand, expressed in NS0 cells.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant. Endotoxin: <0.1EU/ug (LAL)
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human ICOS Ligand. No crossreactivity with recombinant human B7-1, B7-2, B7-H1, B7-H3, recombinant mouse B7-H2 or PD-L2.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
