HNE (4-Hydroxy-2-nonenal) (BSA), ELISA Control
Product Sizes
100ug
£735.00
H6276-05B-100UG
About this Product
- SKU:
- H6276-05B
- Application:
- Western Blot, Control, ELISA
- Extra Details:
- HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display an altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts. Applications: HNE-BSA conjugate can be used to coat ELISA plates (coat 1-10ug/ml) to test antibodies or used a known positive control for ELISA or Western (load 1-5ul/lane). BSA-HNE conjugate is ~65-70kD. Purity: Purified, dialyzed to remove any free 4-HNE. Form: Supplied as a liquid in PBS, pH 7.4, 0.01% benzethonium chloride. Labeled with BSA. Note: BSA-HNE conjugate should only be used as a positive control to optimize the blotting conditions. It is not a representative of the protein bands that might be labeled with the anti-HNE in a given samples. Typically, complex proteins samples, such as liver or brain, will show many bands that are modified with HNE. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Physical State:
- Supplied as a liquid in 10mM Tris-buffer, pH 7.5, 0.01% benzethonium chloride. Labeled with BSA.
- Purity:
- Purified, dialyzed to remove any free 4-HNE.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Sera
