HNE (4-Hydroxy-2-nonenal) (Not for Export EU)
Product Sizes
100ul
£704.00
H6276-05-100UL
About this Product
- SKU:
- H6276-05
- Clonality:
- Polyclonal
- Extra Details:
- HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Free HNE (KLH).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid, 0.05% sodium azide.
- Purity:
- Serum.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes HNE-protein adducts in all species.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
