Histone H4, acetylated (Histone Cluster 4 H4, H4/p, HIST4H4, MGC24116)
Product Sizes
100ug
£876.00
H5110-18S-100UG
About this Product
- SKU:
- H5110-18S
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Histone 4 is a 12-15kD member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on aa2-48, plus aa52-89, and acetylated on at least nine sites. Human histone 4 is 103aa in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78aa identical to Tetrahymena histone 4 over aa2-20 used for immunization. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Synthetic peptide corresponding to AGGKGGKGMGKVGAKRHSK Tetrahymena pyriformis acetylated Histone H4 (P02310).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Acetyl-Histone H4.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
