His-Tag (6-Histidine)
Product Sizes
100ul
£927.00
H5099-15C-100UL
About this Product
- SKU:
- H5099-15C
- translate.label.attr.clone:
- 3H29
- Clonality:
- Monoclonal
- Extra Details:
- Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or C-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Tags enable a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions (1-4). This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins (1-4). Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 using 50-100ng control antigen per well Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- His-tag-fusion protein
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid, 0.09% sodium azide.
- Purity:
- Ascites
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
