Histamine Receptor 3 (H3R) (Not for Export EU)
Product Sizes
100ul
£704.00
H5090-20B-100UL
About this Product
- SKU:
- H5090-20B
- Clonality:
- Polyclonal
- Extra Details:
- Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitter. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors: the H1R, H2R , H3R and H4R receptors. These receptors are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the stimulated release of histamine. The H3 receptor has been shown to be a presynaptic heteroreceptor in nonhistamine- containing neurons in both the central and peripheral nervous systems. H3R (rat/human 445aa, ~93% homology) is primarily expressed in the brain. Several studies using H3 selective agonists revealed that H3R couples to pertussis toxin-sensitive Gi/o protein. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. Optimal dilutions to be determined by the researcher. Control Peptide: H5090-20A Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide 18aa sequence within the extracellular, N-terminus of human HR3 (KLH coupled).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid, neat serum, 0.05% sodium azide, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Human Histamine Receptor 3 (HR3). The immunogen shows no significant sequence homology with other histamine receptors. Species Sequence Homology: Mouse-94%; rat-88%; guinea pig-84%.
- Source:
- human
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
