Helicobacter pylori Urease, Large Subunit Antigen
Product Sizes
100ug
£745.00
H1840-60-100UG
About this Product
- SKU:
- H1840-60
- Application:
- Western Blot
- translate.label.attr.clone:
- 5i57
- Clonality:
- Monoclonal
- Extra Details:
- H. pylori produces large amounts of the enzyme urease, molecules of which are localized inside and outside of the bacterium. Urease breaks down urea (which is normally secreted into the stomach) to carbon dioxide and ammonia. The ammonia is converted to ammonium by accepting a proton (H+), which neutralizes gastric acid. The survival of H. pylori in the acidic stomach is dependent on urease. The ammonia produced is toxic to the epithelial cells, and along with the other products of H. pylori—including proteases, vacuolating cytotoxin A (VacA), and certain phospholipases, damages those cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:16,000 Western Blot: 1:8000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant H. pylori Urease large subunit (60kD).
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.2
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the Urease large subunit in an extract of a cytotoxic H. pylori strain.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
