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Antibodies

Helicobacter pylori, Cag Antigen (HRP)

Product Sizes
100ul
£1022.00
H1840-46C-HRP-100UL
About this Product
SKU:
H1840-46C-HRP
Application:
Western Blot
translate.label.attr.clone:
10J28
Clonality:
Monoclonal
Extra Details:
It is known that H. pylori strains exhibit a significant degree of diversity. The great variability in the H. pylori genome may explain why not all infected individuals suffer from ulcer. Some H. pylori strains contain particular pathogenic genes such as cytokine associated gene A (CagA), while others lack these genes. The CagA protein of H. pylori has been found to be associated with more severe clinical manifestations, such as ulcer disease and gastric cancer. Thus, discrimination between potentially virulent strains may be relevant. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Host:
Mouse
Immunogen:
Recombinant fragments of CA-gene of H. pylori.
Isotype:
IgG
Physical State:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).
Purity:
Purified by Protein G affinity chromatography from ascites
Shipping Conditions:
Blue Ice
Specificity:
Recognizes an epitope localized between Asp562 and Gln795 in the GenBank FJ428215 amino acid sequence.
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody