HSPH1 (Heat Shock Protein 105kD, Antigen NY-CO-25, Heat Shock 110kD Protein, HSP105, HSP110, KIAA0201)
Product Sizes
100ug
£787.00
H1833-02D-100UG
About this Product
- SKU:
- H1833-02D
- Application:
- Western Blot
- translate.label.attr.clone:
- 10K65
- Clonality:
- Monoclonal
- Extra Details:
- The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1, also known as HSP105 and HSP110), exists as three isoforms. HSPH1alpha is 858aa in length and is constitutively expressed. HSPH1beta is 814aa and is induced by heat shock or cell stress. HSPH1 isoform 3 is 817aa and has not been experimentally confirmed. The HSPH1 isoforms have calculated molecular weights of 92-96kD but run at an apparent molecular weight of 105-110kD in SDS-PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. Over aa human 388-505, human HSPH1 has 98% and 96% identity to mouse and rat HSPH1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using HT-29 human colon adenocarcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line and Nb2-11 rat lymphoma cell line trested by heat shock Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lys388-Glu505 of human heat shock protein H1, expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human HSPH1. Species Crossreactivity: mouse and rat.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
