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Antibodies

CXCL1 (GRO/KC)

Product Sizes
100ug
£594.00
G8975-24A-100UG
About this Product
SKU:
G8975-24A
Application:
Western Blot
Clonality:
Polyclonal
Extra Details:
All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant rat GRO/KC is a 7.8kD protein consisting of 72 amino acids including the 'ELR' motif common to the CXC chemokine family that bind to CXCR1 or CXCR2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml (100ul/well). In conjunction with C8297-97A as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant rat GRO/KC. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant rat GRO/KC is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to rat CXCL1, expressed in E. coli.
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes rat CXCL1.
Source:
rat
Storage Conditions:
-20[o]C
Type:
Antibodies:Polyclonal Antibody