Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim)
Product Sizes
50ug
£453.00
G8951-20A-50UG
About this Product
- SKU:
- G8951-20A
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry, Neutralization, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (1ng/ml), a concentration of 0.13-0.2ug/ml of this antibody is required. ELISA (Sandwich): 0.5-2ug/ml (using 100ul/well antibody solution allows detection of antigen at 0.2-0.4ng/well). Western Blot: 0.1-0.2ug/ml (detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions). Immunohistochemistry: 0.25ug/ml with an overnight incubation at 4°C stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
- Host:
- Rabbit
- Immunogen:
- Highly purified (>98%) recombinant human GM-CSF, expressed in E. coli
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
