Glycine Transporter-2 (GLYT2) (Not for Export EU)
Product Sizes
100ul
£704.00
G8168-21-100UL
About this Product
- SKU:
- G8168-21
- Clonality:
- Polyclonal
- Extra Details:
- Glycine is one of the major inhibitory neurotransmitters in the spinal cord and brain stem. Glycine acts on the strychnine-sensitive glycine receptors, a pentameric Cl-channel, to generate inhibitory postsynaptic potentials. Glycine can modulate excitatory neurotransmission as an obligatory co-agonist with glutamate at NMDA-activated glutamate receptors via binding site on the NMDA receptor, which is distinct from the strychnine-sensitive glycine receptor-binding site. Glycinergic synapses are primarily present throughout the spinal cord, brain stem and cerebellum, as well as in the retina and a few other brain regions. Neurotransmitter action is terminated by re-uptake of glycine via specific high affinity transporter protein located at the plasma membrane of neuron and glial cells. The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms (GLYT1 and GLYT2). Form 1 has three other known isoform (GLYT1a, GLYT1b and GLYT1c) which may be produced by alternative spicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and expressed from the same gene. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:10,000-1:50,000. Coat with 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: G8168-25: Glycine Transporter-2, Rat, Control Peptide (GLYT2) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide consisting of 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT2 (KLH).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters. Species Sequence Homology: mouse, and human: 100%
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
