Glutathione Peroxidase 3 (GPX3, GSHPx-3, GPx-3, GSHPx-P, GPx-P, GPXP)
Product Sizes
100ug
£926.00
G8127-13-100UG
About this Product
- SKU:
- G8127-13
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Glutathione Peroxidase 3 (GPX3), also known as Plasma Glutathione Peroxidase, is a 226aa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX3 is secreted into plasma and like GPX1, is an ~100kD homotetramer consisting of four identical 23kD subunits, each containing a selenocysteine residue at the active site. GPX3 exhibits highest levels of mRNA expression in liver (human only), kidney, heart and lung tissues. Mouse GPX3 shares 89% and 99% amino acid identity with human and rat GPX3, respectively. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml tested with lysates of human plasma, mouse kidney tissue, and rat kidney tissue Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human kidney Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-28K: IgG, H&L (HRP) Pab Rb xGt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab Rb xGt I1904-28V: IgG, (HRP) Pab Rb xGt I1904-29A: IgG, (HRP) Pab Rb xGt Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Gly74-Lys226 from mouse GPX3 expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous mouse Glutathione Peroxidase 3 (GPX3). Species Crossreactivity: human and rat. ~15% cross-reactivity with recombinant human (rh) GPX5 and recombinant mouse (rm) GPX1 is observed, and less than 4% cross-reactivity with rhGPX4, rhGPX6, and rmGPX2 is observed.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
