GLUT 9 (Glucose Transporter) (Not for Export EU)
Product Sizes
100ul
£704.00
G3900-91A-100UL
About this Product
- SKU:
- G3900-91A
- Clonality:
- Polyclonal
- Extra Details:
- Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudoegene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leucocytes and brain. Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496 aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000. ELISA: 1:10,000-50,000. Coat ELISA plates at 1ug/ml with G3900-91 (GLUT 9, Human, Control Peptide). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide scorresponding to 21aa near the C-Terminal, cytoplasmic domain of human Glut-9, conjuagted to KLH. Species Sequence Homology: chimpanzee and monkey, 100%, equine, sheep, rabbit; 70%, feline; 55%, chicken; 50%
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human GLUT 9. No significant sequence homology with other gluts.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
