GLUT 3 (Glucose Transporter) (Not for Export EU)
Product Sizes
100ul
£704.00
G3900-24A-100UL
About this Product
- SKU:
- G3900-24A
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. GLUT 2 provides glucose to the liver and pancreatic cells. GLUT 3 is the main transporter in neurons, whereas GLUT 4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 (ECL); Detects a major band at ~45kD. ELISA: 1:10,000-50,000; Coat plates at 1ug/ml with Control Peptide. Optimal dilutions to be determined by the researcher. Control Peptide: G3900-24D: GLUT 3, Human (Glucose Transporter) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGLUT 3) (KLH coupled).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide and 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human GLUT 3 (Glucose Transporter). Species Crossreactivity: Does not recognize mouse/rat GLUT 3. For mouse and rat samples use G3900-25 or G3900-26.
- Source:
- human
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Type:
- Antibodies:Polyclonal Antibody
