GLUT 2 (Glucose Transporter, Insulin Regulatable)
Product Sizes
100ug
£531.00
G3900-15N-100UG
About this Product
- SKU:
- G3900-15N
- Application:
- Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 5D1
- Clonality:
- Monoclonal
- Extra Details:
- GLUT 2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. GLUT 2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug labels 1x10e6 HepG2 human hepatocellular carcinoma cell line Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human brain (medulla), liver, normal pancreas array and pancreatic cancer tissue Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 5% trehalose.
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on human GLUT 2-transfected NS0 cells, but not on control transfectants. Detects GLUT 2 on the surface of the human intestinal cell line Caco-2 in Flow Cytometry and on fixed cells in Immunocytochemistry. Based on Flow Cytometric tests on transfected cells, does not crossreact with human GLUT1 or GLUT3.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
