FOXH1 (Forkhead Box H1, Human Homolog of Xenopus Forkhead Activin Signal Transducer 1)
Product Sizes
100ug
£876.00
F9047-21A-100UG
About this Product
- SKU:
- F9047-21A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- FoxH1 (also known as FAST-1) is a 39kD, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22-30), one FKH (fork head) DNA-binding domain (aa 32-128) and a SMAD-interaction region (aa 273-354). Over aa 164-272, human FOXH1 is 55% identical to mouse FoxH1. Applications: Suitable for use in Western blot, Immunocytochemistry and Direct ELISA Recommended Dilutions: Western blot: 0.1-0.2ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Immunocytochemistr: 10ug/ml to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0ug/ml. The detection limit is~ 0.2 ng/well. Optimal dilutions should be determined by each laboratory for each application. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1 (rhFoxH1; aa 164-272; Accession # O75593). Storage: Lyophilized samples are stable for twelve months from date of receipt when stored at -20 to -70° C. Upon reconstitution, the antibody can be stored at 2-8° C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20 to -70°C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant human rhFoxH1 (aa 164 - 272).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human FoxH1. Species sequence homology: mouse 55%. less than 1% cross-reactivity with rhFoxB2.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
