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Antibodies

FOLR4 (Folate Receptor 4, FRg, FOLBP-4)

Product Sizes
100ug
£876.00
F5750-19B-100UG
About this Product
SKU:
F5750-19B
Application:
Western Blot
Clonality:
Polyclonal
Extra Details:
FOLR4 (Folate receptor 4; also FR4 and folate receptor dH2O and folate binding protein 3) is a 34-36kD member of the folate receptor family of molecules. Based on mouse, it appears to be a marker for subsets of T cells. In particular, and in conjunction with CD25, FR4hiCD25hi expression defines Treg cells, FR4loCD25+ expression pattern identifies effector memory T cells, and a FR4hiCD25- combination characterizes central memory T cells. Mature human FOLR4 is 225aa in length (aa20-244). It contains virtually no identifiable motifs. There is one potential splice variant that shows a five aa substitution for (aa111-116). Over (aa134-220) human FOLR4 shares 72% aa identity with mouse FOLR4. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using A431 human epithelial carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Sheep
Immunogen:
Recombinant protein corresponding to Glu134-Asn210 from human FOLR4, expressed in E. coli.
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity:
Purified by Immunoaffinity chromatography
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human FOLR4. Shows <5% cross-reactivity with recombinant human FOLR1, FOLR2 and FOLR3 in direct ELISA.
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody