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Antibodies

Fluorescein Isothiocyanate (FITC) (PE)

Product Sizes
100ul
£940.00
F5250-17-PE-100UL
About this Product
SKU:
F5250-17-PE
Application:
Immunohistochemistry, FLISA
translate.label.attr.clone:
1.BB.75
Clonality:
Monoclonal
Extra Details:
Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification. Applications: Suitable for use in the detection of FITC labeled antigens and antibodies, as a capture antibody for FITC labeled bioploymers, FLISA, and as a second enzyme for Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice. Affinity Constant: 1.5 x 10e-8 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Host:
Mouse
Immunogen:
FITC-BSA conjugate.
Isotype:
IgG2a
Physical State:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Purity:
Purified by Protein A affinity chromatography. >90% tested by Immunoelectrophoresis.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes free fluorescein and FITC-labeled protein.
Storage Conditions:
4[o]C Do Not Freeze
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody