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Antibodies

Fluorescein Isothiocyanate (FITC) (Biotin)

Product Sizes
100ul
£940.00
F5250-17-BIOTIN-100UL
About this Product
SKU:
F5250-17-BIOTIN
Application:
Immunohistochemistry
translate.label.attr.clone:
1.BB.75
Clonality:
Monoclonal
Extra Details:
Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification. Applications: Suitable for use in the detection of FITC labeled antigens and antibodies, as a capture antibody for FITC labeled bioploymers, ELISA, and as a second enzyme for Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice. Affinity Constant: 1.5 x 10e-8 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Host:
Mouse
Immunogen:
FITC-BSA conjugate.
Isotype:
IgG2a
Physical State:
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.
Purity:
Purified by Protein A affinity chromatography. >90% tested by Immunoelectrophoresis.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes free fluorescein and FITC-labeled protein.
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody