Fluorescein Isothiocyanate (FITC) (Biotin) (Not for Export EU)
Product Sizes
200ug
£704.00
F5250-12B-200UG
About this Product
- SKU:
- F5250-12B
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- NI 239
- Clonality:
- Monoclonal
- Extra Details:
- FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Applications: Suitable for use in Dot Blot, ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: ≥ 1:500 Immunohistochemistry (paraffin): 1:50 - 1;200 Western Blot: ≥ 1:1,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Highly purified fluorescein isothiocyanate isomer 1.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes fluorescein isothiocyanate.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
