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Proteins and Peptides

Fibroblast Growth Factor-19, Recombinant, Human (FGF19, FGFJ)

Product Sizes
25ug
£474.00
F4212-91A-25UG
About this Product
SKU:
F4212-91A
Extra Details:
The FGF family plays central roles during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-19, a member of the FGF family, is a high-affinity heparin-dependent ligand for FGFR4. FGF-19 is expressed during brain development and embryogenesis. Recombinant Human FGF-19 is a 21.8kD protein containing 195 amino acid residues. Recombinant protein corresponding to 195aa from human Fibroblast Growth Factor 19 expressed in E. coli. Molecular Weight: ~21.8kD Amino Acid Sequence: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK Biological Activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 for this effect is 100-150ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1-1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
21.8
Physical State:
Supplied as a lyophilized powder in 5mM Tris, pH 7.6, 75mM sodium chloride. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Purity:
≥95% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins