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Proteins and Peptides

Fibroblast Growth Factor 6, Recombinant, Human (FGF6)

Product Sizes
25ug
£502.00
F4211A-25UG
About this Product
SKU:
F4211A
Extra Details:
FGF-6 is a secreted, heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4. Recombinant Human FGF-6 is an 18.7kD protein consisting of 168 amino acid residues. Recombinant protein corresponding to 168aa from human Fibroblast Growth Factor 6, expressed in E. coli. Swiss/Uniprot: P10767 Biological Activity: Determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is ≤ 0.5ng/ml corresponding to a specific activity of ≥ 2x10e6 units/mg. Amino Acid Sequence: GTRANNTLLD SRGWGTLLSR SRAGLAGEIA GVNWESGYLV GIKRQRRLYC NVGIGFHLQV LPDGRISGTH EENPYSLLEI STVERGVVSL FGVRSALFVA MNSKGRLYAT PSFQEECKFR ETLLPNNYNA YESDLYQGTY IALSKYGRVK RGSKVSPIMT VTHFLPRI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
18.7
Physical State:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.2, with 50mM sodium chloride. Reconstitute with sterile ddH2O to ~0.5mg/ml. Do not vortex. For long term storage it is recommended to
Purity:
≥95% (SDS PAGE, HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins