Fibrillin 1, exon 10, Pro-rich Domain (Not for Export EU)
Product Sizes
250ul
£570.00
F4195-05B-250UL
About this Product
- SKU:
- F4195-05B
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule. Fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils. There is an aberrant expression of fibrillin-1 in Marfan syndrome. Applications: Suitable for use in Immunohistochemistry, ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:2000 Western Blot: 1:200-1:500 Immunohistochemistry: 1:50-1:100 Immunocytochemistry: 1:50-1:100 Immunoprecipitation: 1:100-1:200 Optimal dilutions to be determined by the researcher. Note: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Protein fragment containing proline-rich domain of exon 10 of human fibrillin-1.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS. No preservatives added.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Strongly recognizes human fibrillin-1. Species Crossreactivity: bovine. Does not crossreact with full-length fibrillin-2 secreted by bovine auricular chondroblasts.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
