FKBP1a /FKBP12, 1-108aa, Recombinant, Human (FK506 binding protein 1a, 12kD, PPIase FKBP1A, Rotamase, FKBP1, PKC12)
Product Sizes
100ug
£460.00
F4150-30A-100UG
About this Product
- SKU:
- F4150-30A
- Extra Details:
- FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. Recombinant protein corresponding to aa1-108 with a His-Tag from human FKBP1a, expressed in E. coli. Uniprot/Accession: NP_463460 Molecular Weight: 14.1kD (128aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE Enzyme Activity: Specific activity is >800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Molecular Weight:
- 14.1
- Physical State:
- Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM sodium chloride, 1mM DTT, 10% glycerol.
- Purity:
- ≥95% (SDS-PAGE under reducing conditions and visualized by coomassie blue stain). Endotoxin: ≤1EU/ug (LAL).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
