FADD (Fas-Associated Death Domain Protein, MORT1)
Product Sizes
100ul
£505.00
F0019-51F-100UL
About this Product
- SKU:
- F0019-51F
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- translate.label.attr.clone:
- J1D2
- Clonality:
- Monoclonal
- Extra Details:
- Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1beta-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry/Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blotting: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FADD protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-208 from human FADD, expressed in E. coli
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human FADD.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
