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Proteins and Peptides

EGF, Recombinant, Human, Animal Free (Epidermal Growth Factor, Urogastrone, URG)

Product Sizes
100ug
£338.00
E3410-100UG
About this Product
SKU:
E3410
Extra Details:
EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2kD globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant protein corresponding to human EGF expressed in E. coli. Biological Activity: The EC50, as determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1ng/ml, corresponding to a specific activity of ≥1x10e7 units/mg. Aminp Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
6.2
Physical State:
Supplied as a lyophilized powder. No additives. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Add 0.1% HSA or BSA for long term storage. Do not vortex.
Purity:
≥98% (SDS-PAGE and HPLC) Endotoxin: ≤0.1ng/ug (1EU/ug).
Shipping Conditions:
Ambient
Storage Conditions:
-20[o]C
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins