EP1 Receptor (Not for Export EU)
Product Sizes
100ul
£704.00
E3364-10-100UL
About this Product
- SKU:
- E3364-10
- Clonality:
- Polyclonal
- Extra Details:
- Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid). They include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2. They are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases. Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation. A variety of PGE2- mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described. PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4. These receptors are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR- 7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus. EP1 expression is restricted to kidney (papillary collecting ducts), lung, and stomach (muscularis mucosae layer), spleen, skeletal muscle, testis, and uterus. EP1 has also been localized in neurons of the DRG. An alternatively spliced variant, a 366aa protein carrying a specific change if 49aa from the middle of TM VI to C-terminus, has also been described in rat uterus. The variant is also expressed in the kidney. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot:1:1000-1:5000 (ECL). Detects ~65kD protein in kidney. Optimal dilutions to be determined by the researcher. Control peptide: E3364: EP1 Receptor, Mouse, Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15aa from the mouse EP1 Receptor. Species Sequence Homology: Rat 100%; rabbit and sheep 73%; canine and human 72%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the mouse EP1 Receptor. No significant homology with EP2-4 or other GPCR. Epitope: C-terminal, Extracellular domain
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
