Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph Receptor Tyrosine Kinase A2) (APC)
Product Sizes
100Tests
£806.00
E3360-01E-100TESTS
About this Product
- SKU:
- E3360-01E
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 7H39
- Clonality:
- Monoclonal
- Extra Details:
- EcK, also known as EphA2, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail, which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells or 50ul packed whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Rat
- Immunogen:
- Mouse myeloma cell line NSO-derived recombinant mouse EphA2 Ala22-Ala535 (predicted), Accession # AAA82113
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in saline, ≤ 0.5% BSA and 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein A or G affinity chromatography from culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse EphA2.
- Source:
- mouse
- Storage Conditions:
- 4[o]C Do not freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
