Eco32I (EcoRV)
Product Sizes
2000u
£314.00
E0374-2000U
About this Product
- SKU:
- E0374
- Extra Details:
- 5'-G A T^A T C-3' 3'-C T A^T A G-5' Concentration: 10u/ul, 50u/ul Source: Escherichia coli RFL32 Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used. Storage Buffer: 25mM Tris-HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Eco32I. Ligation Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Eco32I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.21µM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: pUC57 was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1µg of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Enzymes
