Dopamine Receptor, D1a, D1R (Not for Export EU)
Product Sizes
100ul
£704.00
D9011-08-100UL
About this Product
- SKU:
- D9011-08
- Clonality:
- Polyclonal
- Extra Details:
- Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. Detects 2 proteins of 50 and 75kD in solubilized hippocampal membranes. The 75kD band was not visible in striatum (2). ~100-400ug protein/lane should be loaded to visualize the 75kD band. ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml of Control Peptide. Optimal dilutions to be determined by the researcher. Control Peptide: D9011-10: Dopamine Receptor, D1a, D1R, Rat (Control Peptide) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide 9aa sequence of rat D1R receptor within the C-terminus of the cytoplasmic domain (KLH coupled). Species Sequence Homology: human: 88%; porcine: 77%
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the rat dopamine receptor D1a. No significant sequence homology with other dopamine receptors (D1B, D2-D5).
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
